Abstract: Background: Cardiovascular disease (CVD) remains the leading cause of death in US. US women Veterans (WV) are a growing and at high-risk population for CVD but understudied and under-represented in CVD research. This study investigated the potential ethnic and racial differences in the prevalence of traditional CVD risk factors among US WV during 2000-2019. Methods: The national Veterans Affairs (VA) electronic health records were utilized to assess prevalence of diabetes (DM), hypertension (HTN), hyperlipidemia (HL), and smoking, among US WV who visited a VA facility from 1/1/2000 to 12/31/2019. All diagnoses were based on International Classification of Disease (ICD) 9th and 10th version diagnostic codes. Results: Number of WV who visited a VA steadily increased from 80,707 in 2000 to 739,309 in 2019. Prevalence of all risk factors increased significantly (p<0.01) over the study period. Significant differences in the prevalence of each risk factor were also observed based on race and ethnicity. Conclusion: These data suggest alarming increase in the prevalence of CVD risk factors for US WV during 2000-2019 period, and significant racial and ethnic differences in these risk factors. Timely actionable interventions and research is required to improve cardiovascular health of US WV.